利用正交设计优化米根霉Rh0295发酵L-乳酸的培养基  

Optimized of culture medium by orthogonal experiment of Rhizopus oryzae Rh0295 to ferment L-lactic acid

作　　者：刘磊[1] 李元媛[2] 高媛[2] 蔡俊[2] 

机构地区：[1]湖北工业大学理学院,湖北武汉430068 [2]湖北工业大学发酵工程省部共建教育部重点实验室

出　　处：《安徽农学通报》2009年第10期37-38,157,共3页Anhui Agricultural Science Bulletin

基　　金：湖北省教育厅重点攻关项目;项目编号:2003A008;2003BC17

摘　　要：通过单因素试验,确定了米根霉Rh0295发酵生产L-乳酸的最适发酵碳源、氮源和磷源分别为大米淀粉、(NH4)2SO4及KH2PO4。采用3因素3水平的正交试验确定了3者对L-乳酸发酵的影响。其顺序为大米淀粉>(NH4)2SO4>KH2PO4,优化了它们的最佳用量分别为:大米淀粉10%、(NH4)2SO40.15%、KH2PO40.015%。According to the single factor experiment, the best carbohydrate resource, nitrogen resource and phosphorus resource of Rhizopus oryzae Rh0295 to produce L-lactic acid by fermenting were determined. They were starch of rice, （ NH4 ） 2SO4 and KH2PO4 respectively. Through orthogonal experiment L9, the influence order of them was starch of rice〉（ NH4 ） 2SO4〉KH2PO4. The best dosage of them were optimized. They were 10%,0.15% and 0.015% respectively.

关 键 词：L-乳酸 米根霉Rh0295 发酵 培养基优化 

分 类 号：O623.611[理学—有机化学]