渗透化细胞转化生成苯乳酸的研究  

Study on the Phenyllactic Acid Production by Permeabilized Cells

作　　者：沐万孟[1] 刘凤丽[1] 张涛[1] 江波[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室

出　　处：《食品与发酵工业》2009年第3期19-22,共4页Food and Fermentation Industries

基　　金："十一五"国家863项目(2006AA10Z334)资助

摘　　要：Lactobacillus sp.SK007乳酸脱氢酶是催化苯丙酮酸转化生产苯乳酸的关键酶。实验中研究了渗透化剂对于Lactobacillus sp.SK007乳酸脱氢酶表观酶活的影响,优化了细胞渗透化处理过程;研究了pH和温度对于渗透细胞和粗酶液乳酸脱氢酶表观酶活及稳定性影响,优化了Lactobacillus sp.SK007渗透化细胞转化生产苯乳酸的工艺。渗透化过程:以2%乙醚为渗透剂,渗透时间2min,渗透化温度30℃;渗透化细胞转化生产苯乳酸工艺:苯丙酮酸底物浓度为5g/L,反应温度40℃,pH为6.0,转化时间为4h,PLA的产量达到3.38g/L。Lactate dehydrogenase （LDH） is responsible for the production of phenyllactic acid from phe nylpyruvic acid in Lactobacillus sp. SK007. The effect of cell permeabilization on apparent activity of Lactobacillus sp. SK007 LDH was studied. The permeabilization process was optimized. In addition, the effects of pH and temperature on apparent activity of LDH and enzymatic stability were researched, and the phenyllactic acid production process by permeabilized cells was also optimized. The optimized permeabilization process was 2% aether, the treated time and temperature for 2 rain and 30 ℃, respectively. The optimized conditions of phenyllactic acid production by permeabilized cells was 5 g/L phenylpyruvic, temperature at 40 ℃, pH 6.0, reaction time 4 h, which allows the maximum phenyllactic acid productivity of 3.38 g/L.

关 键 词：LACTOBACILLUS sp.SK007 乳酸脱氢酶 渗透化 苯乳酸 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]