葡萄酒中赭曲霉素A测定方法的研究  

Comparison of Methods for the Determination of Ochratoxin A in Wines

作　　者：康健[1,2] 钟其顶[2] 熊正河[2] 肖冬光[1] 张辉 

机构地区：[1]天津科技大学,天津300457 [2]中国食品发酵工业研究院,北京100027 [3]中粮酒业有限公司,北京100005

出　　处：《食品与发酵工业》2009年第3期153-157,共5页Food and Fermentation Industries

摘　　要：采用固相萃取小柱对葡萄酒中的赭曲霉素A(OTA)进行浓缩处理,选择高效液相荧光法和超高效液相质谱联用法,分别测定葡萄酒中的OTA含量。采用HPLC-FLD法测定葡萄酒中OTA时,线性良好,R2=0.9997;经固相萃取小柱浓缩后,测定方法检出限可达到0.002μg/L;回收率为85.7%～102.6%;RSD(n=5)为4.5%。通过HPLC-FLD和UPLC-MS/MS测定OTA方法对比发现HPLC-FLD方法具有更好的灵敏度和检出限。In accordance with the nature of wine and OTA, we performed sample clean--up utilizing solid --phase extraction （SPE）. The detection of wine OTA was accomplished using high--performance liquid chromatography （HPLC） combined with fluorescence detection （FLD）, and ultra performance liquid chromatography （UPLC） combined with Tandem Quadrupole （MS/MS）. Detection was validated by the clear linearity （Rz=0. 999 7） and the detection limit of OTA in wine were 0. 004 g/L after concentration; recovery rate was 85.7%-102.6 %, and precision （RSD=4.5%, n=5）. The comparison of HPLC-FLDandUPLC -MS/MS in terms of sensitivity and detection limit led to the conclusion that HPLC-FLD offered better results in the sub-ppb concentration level and higher sensitivity.

关 键 词：赭曲霉素A 葡萄酒 高效液相-荧光检测器 超高效液相色谱/质谱联用 

分 类 号：TS262.6[轻工技术与工程—发酵工程]