里氏木霉Rut C-30产非淀粉多糖酶发酵条件的研究  

Study on the Fermentation Conditions of Producing Non-starch Polysaccharides Enzymes in Trichoderma reesei Rut C-30

作　　者：单达聪[1] 王雅民[1] 张董燕[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《食品与发酵工业》2009年第4期111-115,共5页Food and Fermentation Industries

摘　　要：在接种量与培养温度分别为8%和30℃条件下,采用正交试验表L9(34)设计对培养基组成、发酵时间、培养基湿度和发酵容器4个试验因子进行产酶影响力排序和条件组合优化,同时分析各指标间相关性。结果:对纤维素酶,培养基组成>发酵时间>发酵容器>初始湿度,最优组合酶活力456.3u/g;对木聚糖酶,发酵时间>培养基组成>发酵容器>初始湿度,最优组合酶活力5529.9u/g;对β-甘露聚糖酶,发酵容器>培养基组成>初始湿度>发酵时间,优化组合酶活力367.6u/g;对果胶酶,培养基组成>发酵容器>发酵时间>初始湿度,最优组合酶活力为544.6u/g;培养基发酵失重与β-甘露聚糖酶活力极显著正相关(r=0.811,P<0.01)、纤维素酶与木聚糖酶活力显著正相关(r=0.724,P<0.05)。结论:通过改变发酵条件可调控该菌株产饲用NSP酶谱的酶活水平与比例,指标之间相关性可用于监测发酵过程中的酶活力状态。该菌株较适合作为饲用NSP复合酶制剂生产的菌株。To optimize the solid-state fermentation conditions of producing fodder non-starch polysaccharides enzymes in Trichoderma reesei Rut C-30. The inoculation and the culture temperature was in 8% and 30℃, optimized and ordering for the medium, the duration, the Initial humidity and container with the orthogonal table of L9 （34）. And the same time, correlation among index was analyzed. Result showed ： for the cellulase,medium 〉 duration 〉 conainer 〉 Initial humidity ; the enzymes activity of optimal combination was 456.3 u/g ; for the xylanase, duration 〉 medium 〉 container 〉 Initial humidity, the enzymes activity of optimal combination was 5529.9 u/g; for the β-mannase, container 〉 medium 〉 Initial humidity 〉 duration, the enzymes activity of optimal combination was 367.6u/g; for the pentinase, medium 〉 container 〉 duration 〉 Initial humidity, the enzymes activity of optimal combination was 544.6 u/g. The results of Index correlation： positive correlation was statistically significant between the weight loss of the medium and the activity of the β-mannase（r =0. 811 ,P 〈0.01 ） and between the activity of the cellulase and the xylanase （ r = 0. 724,P 〈 0.05）. Conclusion ： It is possible to adjust the level and ratio of zymogram by changing the ferment notation condition, when we want to produce the fodder NSP enzymes in this strain. It is also possible to estimate the activity state of enzymes by the correlation among index. This strain is better in terms of suitability for producing the fodder NSP compound enzymes.

关 键 词：里氏木酶Rut C-30 固态发酵 非淀粉多糖酶 发酵条件 影响力 相关性 

分 类 号：TQ925[轻工技术与工程—发酵工程] S816.7[农业科学—饲料科学]