Rhizobium sp.N613胞外多糖流变性及毒性研究  

Study on rheological property and toxicity of exopolysaccharide from Rhizobium sp.N613

作　　者：任盛[1] 程红兵[1] 陈艳丽[1] 谢红 赵良启[1] 

机构地区：[1]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [2]山西省食品药品监督管理局,山西太原030012

出　　处：《食品工业科技》2009年第5期116-119,123,共5页Science and Technology of Food Industry

基　　金：山西省自然科学基金资助项目(2006011073);山西高校科技研究开发资助项目(20051203)

摘　　要：主要研究了浓度、剪切力、温度、pH、盐离子浓度、加热时间、冻融变化、微波及超声波处理等对Rhizobium sp·N613(REPS)胞外多糖粘度的影响。实验结果表明:REPS溶液的粘度随着浓度的增加而增加,当浓度达到0·9%时,其粘度为41·5mPa·s;REPS溶液为非牛顿流体,具有假塑性;REPS的最佳溶解温度为70℃,加热时间以1·5h为宜;pH、冻融变化、短时间微波和超声波处理对REPS溶液的粘度影响较小;REPS有良好的耐盐稳定性和抗降解性能;0·6%REPS溶液的吸光度为0·758,透光率为17·78%,在水中的溶解度为31·67%。此外,还进行了REPS的特性粘度、急性毒性测试和蓄积毒性实验,结果表明,该多糖具有粘度高、无毒性的特点,在食品加工等领域有着广泛的应用前景。The influences of concentration, shear force,time in hot water, pH, freezing-thawing, concentration of salt ionic,microwave, ultrasonic on viscosity of REPS were studied.The result showed that the viscosity of REPS solution,increased with the increment of concentration,the viscosity was 41.5mPa ·s when concentration reached to 0.9% ,it is non-Newton fluid, and the solution had pseudo plasticity.The best temperature and time of REPS dissolved in hot water were 70℃ and 1.5h.pH,freezing-thawing, antiseptic, short time treat with micro-wave and ultrasonic had little effect on the fluid of REPS.REPS had better stabilization in salt-resistance and antidegradation.The absorbency of the 0.6% REPS solution was 0.758, and the light transmittance was 17.78%, the solubility in water was 31.67%.At the same time, the thesis carried out the test of the viscosity, acute toxicity and accumulative toxicity of REPS.It was concluded that REPS had high viscosity and no toxicity.So it had potential applied prospects in food manufacture.

关 键 词：胞外多糖 流变性 毒性 

分 类 号：TS201.3[轻工技术与工程—食品科学]