响应面法优化胆汗中胆红素提取条件的研究  

Study on optimizing the extraction of bilirubin in bile by response surface methodology

作　　者：李良玉[1] 杨楠[1] 李玲 张丽萍[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]大庆乳品有限公司,黑龙江大庆163311

出　　处：《食品工业科技》2009年第5期242-244,共3页Science and Technology of Food Industry

基　　金：黑龙江省科技厅“十一五”重点课题(GB06B403-3)

摘　　要：以猪胆汁为原料,利用响应面法优化胆汁中胆红素的提取条件,研究水解pH、水解温度、水解时间、萃取pH等单因素对提取胆红素的影响,通过SAS8·2统计分析系统对二次旋转实验结果进行分析,结果显示:水解pH10·58,水解温度95·75℃,水解时间21·89min,萃取pH5·23,最大提取率为70·05%。Taking porcine bile as the raw material,the response surface methodology was used in optimizing the extraction of bilirubin.The influence of the hydrolysis pH, the hydrolysis temperature, the hydrolysis time, the extraction pH on extraction of bilirubin were studied,and SAS8.2 statistics analysis system was used in analyzing the result of experiment.The result showed that when set the hydrolysis pH at 10.58,the hydrolysis temperature at 95.75℃,the hydrolysis time at 21.89min,the extraction pH at 5.23,the extraction ratio of bilirubin was 70.05%.

关 键 词：胆红素 响应面法 快速法提取 

分 类 号：TS201.2[轻工技术与工程—食品科学]