马铃薯叶中茄尼醇的RP-HPLC分析  

RP-HPLC method for determining the content of solanesol in the extracts of potato leaves

作　　者：杨玲娟[1] 张继[2] 陈桐[3] 徐小龙[2] 马俊义[2] 孙学刚[3] 王自社[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水730070 [2]西北师范大学生命科学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《食品工业科技》2009年第5期330-331,340,共3页Science and Technology of Food Industry

基　　金：教育部高等学校科技创新工程重大项目培育基金资助项目(706056)

摘　　要：目的:建立测定马铃薯叶中茄尼醇的反相高效液相色谱(RP-HPLC)分析方法。方法:采用Agilent 1100 HPLC,XDB-C18色谱柱(250mm×4·6mm,5μm),流动相V(甲醇)∶V(乙醇)=45∶55等度洗脱,洗脱流速1·0mL/min,柱温35℃,检测波长211nm,保留时间约8·750min。结果;茄尼醇质量浓度在6·125～196μg/mL(R=0·9999)范围内线性关系良好,平均加样回收率(n=9)为99·04%,RSD=1·87%。通过与传统提取方法比较,确定最佳的提取方法为微波辐射法。结论;该方法简便、快速、准确、重复性好,适用于马铃薯叶中茄尼醇的含量测定。Objective： A sensitive RP-HPLC method was established for determining the content of solanesol in the extracts of potato leaves.Methods ：The Agilent 1 100 HPLC system,A I.D.250mm × 4.6mm, particle size 5μm Agilent XDB-C18 column,was operated at a column temperature of 35℃.The mobile phase was V（ carbinol） ：V（ ethanol） = 45：55 at a flow rate of 1.0mL/min.211 nm was set as the UV detection wavelength for solanesol, retention time was probable 8.750min.Results： The experimental results showed a good linear relationship within 6.125 - 196μg/mL （ R =0.9999） ,the average recoveries（ n = 9） were 99.04%, RSD = 1.87/%.Compared with the conventional methods of extraction, the optimum extraction method was Microwave-assisted extraction.Conclusion： The method was rapid,simple,accurate and good reproducibility, it was suitable for the determination of solanesol in the extracts of potato leaves.

关 键 词：茄尼醇 反相高效液相色谱 马铃薯叶 微波辐射 

分 类 号：TS207.3[轻工技术与工程—食品科学]