海带提取物的鉴定及抗菌活性的研究  

Antibacterial Activities and Identification of Different Solvent Extracts of Kelp Powder

作　　者：王力[1] 董乐[1,2] 杨秋明[1] 冯珍鸽[1] 蔡慧农[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]泉州师范学院化学与生命科学学院生物系,福建泉州362000

出　　处：《食品科学》2009年第11期58-61,共4页Food Science

基　　金：国家自然科学基金项目(20871054);福建省教育厅基金项目(2007F5076);福建省自然科学基金高校专项基金项目(2007J0369)

摘　　要：本实验以海带为原料,用四种不同的溶剂(蒸馏水、甲醇、正丁醇、乙酸乙酯)对其进行萃取,并通过正交试验得到海带各种提取物的最佳提取工艺,用高效液相色谱对这四种提取物结构进行初步表征,结果表明:海带粉提取物含有多种糖类化合物;对这四种提取物进行抗菌活性的研究表明这四种提取物在一定浓度下对枯草芽胞杆菌、大肠杆菌、四联球菌和金黄色葡萄球菌均有较明显的抑制作用,在稀释后仍然对上述菌种显示出一定的抑制作用。其中以乙酸乙酯提取物对枯草芽孢杆菌抑菌效果最明显。Kelp powder was extracted with 4 different solvents, including distilled water, methanol, n-butanol and ethyl acetate, and orthogonal test design was adopted to optimize the extraction technology, respectively. The primary structures and antibacterial activities of obtained 4 extracts were analyzed by HPLC and filter paper disc diffusion method, respectively. Glucose was identified in he water extract and the ethyl acetate extract, in which fructose was also identified, trehalose was identified in the methanol extract and the n-butanol extract, in which sucrose was also identified by RP-HPLC analysis. All the extracts showed significant higher inhibitory activity against Bacillus subtilis, Escherichia coli, Gaffkya tetragena and Staphylococcus aureus, and their inhibitory effects could be observed markedly at high dilution. Among the four solvent extracts, the ethyl acetate extract presented the strongest sensitivity to Bacillus subtilis.

关 键 词：海带粉 提取物 抑菌作用 抗菌活性 

分 类 号：R285.6[医药卫生—中药学]