莲原花青素抗黑色素瘤的研究  

in vivo and in vitro Inhibitory Activities of Proanthocyanidins from Lotus Seedpod (LSPC) and Lotus Seed Hulls (LSHPC) against Melanoma B16 in Mice

作　　者：周密[1] 段玉清[2] 王文兵[1] 李金凤[2] 徐菲菲[2] 谢笔钧[3] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2009年第11期223-226,共4页Food Science

基　　金：国家自然科学基金项目(30270938);江苏省高校自然科学研究计划项目(05KJB550011);江苏大学人才基金项目(05JDG013)

摘　　要：目的:探讨莲房和莲子壳中原花青素对黑色素瘤B16生长的影响。方法:采用MTT法测定莲房和莲子壳原花青素对B16细胞的体外抑制作用;并借助显微技术观察B16细胞形态;以荷黑色素瘤的C57BL/6J小鼠为体内研究对象,通过瘤体积和瘤重测定,探讨体内抑瘤作用,采用黄嘌呤氧化酶法和硫代巴比妥酸法测定荷瘤鼠血清中超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:莲房和莲子壳中原花青素体外对黑色素瘤B16细胞均有较强的抑制作用,抑制率分别为87.4%和78.2%,均使细胞的膜破损,细胞形态改变;体内能显著减小瘤体积和减轻瘤重,抑瘤率分别为55.3%和46.7%,并能有效降低荷瘤小鼠血清中MDA含量,提高SOD活力。结论:莲房和莲子壳中原花青素对黑色素瘤均具有较强的抑制作用。Proanthocyaidins were extracted from lotus seedped （LSPC） and lotus seed hulls （LSHPC） with 1% NaHSO3 solution by boiling for 15 rain and purified with AB-8 macroporous resin. After incubated with culture medium containing different concentrations of the proanthocyaidins from LSPC and LSHPC for 48 h and 72 h, the viability of mouse melanoma B 16 cells was measured by MTT assay, and their morphology was observed under inverted microscope and fluorescence microscope after 72 h incubation with medium containing the two proanthocyaidins at 100 μg/ml. The in vivo inhibitory effect against the proliferation of mouse melanoma B16 cells was investigated by using C57BL/6J mice, and the levels of superoxide dismutase （SOD） and malonicaldehyde （MDA） in mouse serum were determined by xanthine oxidase assay and thiobarbituric acid assay. Results showed that both the proanthocyaidins from LSPC and LSHPC were able to significantly inhibit proliferation of B 16 cells in vitro, and the inhibition rates were 87.4% and 78.2%, respectively. The morphological alterations of melanoma B16 cell membrane damage caused by them were observed. Both the two proanthocyaidins could significantly reduce the volume and mass increases of melanoma B 16 in in vivo experiments, and their inhibition rates at 120 mg/kg bw against the mass of melanoma B 16 reached 55.3 % and 46.7 %, respectively. Serum MDA level was reduced by administration of the proanthocyaidins from LSPC and LSHPC accompanied with the enhancement in serum SOD activity.

关 键 词：莲房 莲子壳 原花青素 黑色素瘤B16 抑制作用 

分 类 号：R739.5[医药卫生—肿瘤]