酿酒酵母胞浆中乙醛脱氢酶的提取和研究  

Extraction of and Research on Cytosolic Aldehyde Dehydrogenase from Saccharomyces cerevisiae

作　　者：周百灵[1] 高秀峰[1] 吴晓婷[1] 赵绪光[1] 李永生[2] 王健[2] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041 [2]四川大学化学工程学院,四川成都610065

出　　处：《酿酒科技》2009年第6期28-30,共3页Liquor-Making Science & Technology

基　　金：四川大学振兴计划科研启动基金(No.0082204127092)

摘　　要：利用超声波破碎、硫酸铵分级盐析以及DEAE-32阴离子交换层析等方法提取了酿酒酵母胞浆内的乙醛脱氢酶(ALDH),并对其酶学性质进行了研究。结果表明,经过DEAE-32交换层析后得到的ALDH酶液,纯化倍数为11,产率为17%,酶的最适反应温度为25℃,最适pH在7.0～7.5之间。Cytosolic Aldehyde dehydrogenase （ALDH）was extracted from Saccharomyces cerevisiae by using ultrasonication, ammonium sulfate fractionation and ion exchange chromatography on DEAE-32, and the properties of ALDH were studied. The results showed that the yield of 11-fold purified ALDH by DEAE-32 exchange chromatography is 17 %, the optimum reaction temperature of ALDH is at 25℃, and the optimum pH value is between 7-7.5.

关 键 词：微生物 酿酒酵母 乙醛脱氢酶 纯化 

分 类 号：TS261.11[轻工技术与工程—发酵工程]