降解秸秆的纤维素酶产生菌的筛选及产酶条件研究  

Screening of Cellulase Producing Strain for Straw Degradation and Optimization of Fermentation

作　　者：武峥[1] 张迎君[1] 周心智[1] 

机构地区：[1]重庆市农业科学院果树研究所,重庆江津402260

出　　处：《纤维素科学与技术》2009年第2期20-26,共7页Journal of Cellulose Science and Technology

基　　金：重庆市科委自然科学基金项目(2006BB1268)

摘　　要：从土壤、霉变的农产品和实验室保存的真菌中筛选到15株产纤维素酶的菌株,其中绿色木霉T206产酶能力最强。利用液体发酵,研究了碳源、氮源、接种量、起始pH值、培养时间等对菌株产酶的影响,以及该菌株所产纤维素酶的种类。在最适条件下菌株培养96h后,羧甲基纤维素酶活(CMCA)最高达到7654.33U,是优化前酶活的1.7倍,滤纸酶活(FPA)达到1675.12U。15 strains with performance to hydrolyze cellulose were screened from soil, rotten produce and preserved fungi in our laboratory. A Trichoderma Viride T206 with relatively high cellulase activity was isolated. The isolated sample was fermented in liquid state after isolation, and its fermentation conditions （carbon source, nitrogen source, inoculating volume, initial pH and cultivating time） and enzyme component were studied. Under optimum condition, its hydrolysable efficiency upon carboxymethyl cellulose and filter paper reached 7654.33 U and 1675.12 U.

关 键 词：绿色木霉 纤维素酶 筛选 产酶条件 

分 类 号：S188[农业科学—农业基础科学]