DHA纳米结构脂质载体的制备和表征  

Preparation and characterization of dha-loaded nanostructured lipid carriers

作　　者：徐弟[1,2] 夏强[1,2] 徐峰[1,2] 唐金国[1,2] 

机构地区：[1]东南大学生物科学与医学工程学院,南京210096 [2]东南大学苏州市生物医用材料与技术重点实验室,苏州215123

出　　处：《食品科技》2009年第6期29-32,共4页Food Science and Technology

基　　金：科技部国际科技合作项目(2008DFB50060);美国可口可乐公司国际合作项目

摘　　要：目的:制备DHA纳米结构脂质载体(DHA-NLC)并考察其理化性质。方法:采用高压均质法制备DHA纳米结构脂质载体;考察了高压均质循环次数对纳米粒制备的影响并进行优化;测定其粒径大小和分布、Zeta电位和负载量,考察了纳米粒的时间、温度、离心稳定性。结果:制得DHA-NLC的平均粒径110 nm,多分散指数(PI)值为0.562,Zeta电位为-20.1 mV,负载量1.81%;DHA-NLC置于5℃和25℃条件下存放1个月粒径变化很小,且高速离心稳定性很好。结论:高压均质法优化制备的DHA-NLC稳定性很好,重复性高。DHA-Ioaded nanostructured lipid carriers（DHA-NLC） were prepared by high pressure homogenization method. The influence of high pressure homogenization cycles on the preparation of DHA-NLC was explored. The mean size, Polydispersity Index（PI）, Zeta potential and drug loading were characterized. The results were： the mean size of DHA-NLC was 110 nm. The PI was 0.562, The Zeta potential was -20.1 mV and DHA loading was 1.81%. The DHA-NLC remained stable stored at 5 ℃ and 25 ℃ for one month. After centrifugation, there was no change to mean size of DHA-NLC.

关 键 词：DHA 纳米结构脂质载体 高压均质法 

分 类 号：S985.22[农业科学—捕捞与储运]