酶标仪法快速评价香兰素的抑菌活性  

Rapid Evaluating of Antimicrobial Activity of Vanillin with the Microplate Reader in 96-cell Plate

作　　者：陈默[1,2] 王志伟[1,2] 胡长鹰[3] 吴希阳[3] 王平利[2] 

机构地区：[1]江南大学包装工程系,江苏无锡214122 [2]暨南大学包装工程研究所,广东珠海519070 [3]暨南大学食品系,广东广州510632

出　　处：《食品与发酵工业》2009年第5期63-66,共4页Food and Fermentation Industries

基　　金：广东省自然科学基金项目(07005952);珠海市科技计划项目(PB20051013)

摘　　要：采用96孔板在酶标仪上测定了大肠杆菌(E.coli)、金黄色葡萄球菌(S.aureus)、李斯特菌(L.mono)、沙门氏菌(S.enteritidis)和宋内氏菌(Sh.Sonnei)在不同浓度香兰素存在下的光学密度(optical density,OD)值,并绘制其OD-t生长曲线。受试菌生长曲线显示香兰素对E.coli,S.aureus,L.mono,S.enteritidis和Sh.Sonnei的最低抑菌浓度分别为1.6mg/mL,2.2mg/mL,1.0mg/mL,1.2mg/mL和1.6mg/mL。The Microplate Reader is used to detect the antimicrobial activity of vanillin against Escherichia coli, Staphylococcus aureus , Listeria monocytohenes , Salmonella enteritidis , and Shigella sonnei in a sterile 96-well microtitre plate. The growth curves of tested bacteria indicate that vanillin exhibits certain antimiero- bial effect against E. coli, S. aureus, L. mono, S. enteritidis, and Sh. sonnei, with minimum inhibitory concentration （MIC） values of 1.6mg/mL, 2.2 mg/mL, 1.0 mg/mL, 1.2 mg/mL and 1.6 mg/mL, respectively. This method is proved to be economical, rapid and effective for detecting the efficiency of antimicrobial.

关 键 词：酶标仪 抑菌活性 香兰素 抑菌剂 生长曲线 

分 类 号：S482.2[农业科学—农药学]