发酵法生产L-异亮氨酸的溶氧控制策略  

Microbial production of L-isoleucine by dissolved oxygen control strategy

作　　者：彭志坚[1,2] 房峻[1,2] 李江华[1,2] 堵国成[1,2] 陈坚[1,2] 宁健飞 蔡立明 

机构地区：[1]江南大学工业生物技术教育部重点实验室 [2]江南大学生物工程学院,无锡214122 [3]无锡晶海氨基酸有限公司

出　　处：《工业微生物》2009年第3期11-16,共6页Industrial Microbiology

基　　金：国家高技术研究发展计划(863)项目资助(No.2007AA02Z229)。

摘　　要：研究了溶氧对Brevibacterium lactofermentation分批发酵生产L-异亮氨酸(Ile)的影响,提出了前10h恒700r/min以维持溶氧在35%以上,10h后调至600r/min以维持溶氧在15%～20%的两阶段供氧控制模式。与对照相比,获得了较高的产率(0.094g/g)和糖耗速度(4.76g/L·h),在较短时间内(52h)获得较高的Ile产量(23.3g/L),比结果最好的单一搅拌转速(600r/min)提高11.6%。生产强度(0.448g/L·h)比恒定搅拌转速(500、600、700、800r/min)控制下的过程分别提高了83.6%、28.7%、44.9%、35.7%。最后采用代谢通量分析对该结果产生的原因进行了定量解释。The effect of dissolved oxygen （DO） on L-isoleucine production by Brevibacterium lactofermentation was investigated in batch fermentation process. A two-stage DO control strategy was developed, in which the agitation speed was controlled at 700 r/min during in the first 10 h to achieve a DO level up to 35%, and then switched to 600 r/min after 10 h to maintain a DO level at 15% --20 %. A relative high yield （0. 094 g/g） and glucose consumption rate （4.76 g/L·h） were obtained. The high isoleueine production （23.3 g/L） was achieved in a relative short period （52 h）, and it was increased by 11.6o,6 compared to the highest value ofby using single agitation speed （600 r/min）. According to the results in constant agitation speed （500, 600, 700, 800 r/min）, the productivity （0. 448 g/L·h） was increased by 83.6 %, 28.7%, 44.9 %, and 35.7 %, respectively. Finally, the reason for the increase of isoleucine production with the proposed two-stage DO control strategy was quantitatively described in detail by analysis of metabolic flux distribution in metabolic network of isoleucine production by Brevibacterium lactofermentation.

关 键 词：异亮氨酸 BREVIBACTERIUM lactofermentation 分批发酵 溶氧控制 代谢通量 

分 类 号：TQ922.9[轻工技术与工程—发酵工程] TQ929