无花果曲霉发酵生产β-葡萄糖苷酶的动力学分析  

Kinetic analysis of β-glucosidase production from Aspergillus ficuum

作　　者：姚卫蓉[1] 张玲[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《工业微生物》2009年第3期30-33,共4页Industrial Microbiology

基　　金：教育部111项目B07029和PCSIRT0627支持。

摘　　要：在7L生物反应器的分批发酵中,通过对无花果曲霉UV-29液态发酵茵丝体的生长、基质消耗(以总糖计)及β-葡萄糖苷酶产生的特性研究,发现总糖是无花果曲霉生长的限制性基质;β-葡萄糖苷酶的增长趋势明显滞后于细胞生长的增长趋势,其发酵过程属于部分相关模型,即Ga-den提出的Ⅱ型发酵;基于Logistic方程,建立了发酵动力学模型,同时对实验数据与模型进行了验证比较,模型计算值与实验数据拟合良好。在7L生物反应器的最大茵体生物量(干重)达到1.17g/100mL,β-葡萄糖苷酶最高酶活达到22.25IU/mL。The fermentation process of Aspergillus ficuum UV-29 was studied in a 7 L batch system. A kinetic model was proposed based on the Logistic equation for microorganism growth （calculated by biomass concentration）, β-glucosi- dase activity and substrate consumption（calculated by total carborlhyclrate）. With the evaluated model parameters, the model appeared to provide a reasonable description for the fermentation process under various conditions. The fermentation in biorcactor was successfully performed with a maximal biomassI concentration （dry mass） of 1.17 g/100mL and a maximal β-glucosidase activity of 22.25 IU/mL.

关 键 词：无花果曲霉 Β-葡萄糖苷酶 动力学模型 

分 类 号：TQ925[轻工技术与工程—发酵工程]