嗜热真菌DSM10635生产耐热木聚糖酶的小试研究  

High-yielding thermostable xylanase production by Thermomyces lanuginosus DSM10635

作　　者：农旭华[1] 张继泰[1] 王玉瑛[1] 王晓琼[1] 熊海容[1] 

机构地区：[1]中南民族大学生命科学学院,湖北武汉430074

出　　处：《工业微生物》2009年第3期62-66,共5页Industrial Microbiology

基　　金：国家民委自然科学基金(08ZN04);中南民族大学自然科学基金(YZZ06016);人事部留学人员择优资助优秀项目(2008-86)。

摘　　要：应用嗜热真茵Thermomyces Lanuginosus DSM10635,采用固体发酵的方法探索耐热木聚糖酶的优化生产条件。在研究玉米芯,玉米皮,玉米秆,麸皮,松树屑,桦树屑等不同底物,在不同温度、玉米芯颗粒大小以及料水比条件下培养比较酶产量后,发现该嗜热真菌产耐热木聚糖酶的最佳底物为玉米芯或玉米皮,最佳培养温度为50℃～55℃,在加水量为1份玉米芯:2.8份水,玉米芯的颗粒直径大约为1mm时产酶量最高。实验结果显示,嗜热真菌DSM10635在优化后的培养条件下木聚糖酶产量可达到12525.80IU/g玉米芯。The thermostable xylanase production by Thermomyces lanuginosus DSM10635 was carried out using solid substrate fermentation and the influences of substrates, temperatures, corncob particle diameter, water addition on the xylanase production were investigated. By comparing the substrates of corncob, corn husk, corn stalk, pine powder, birch powder, and wheat bran, corncob was a good substrate for xylanase production. The highest xylanase productivity was achieved at 50℃--55℃, particle diameter about i mm, and dry corncob/water ratio as 1/2.8 for corncob solid-state fermentation. Under the optimum conditions, the highest yield of xylanase was obtained as high as 12525.80 IU/g dry weight of corncob.

关 键 词：嗜热真菌 木聚糖酶 酶活力固体发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]