响应面法优化纳豆激酶液体发酵条件的研究  

Optimization of Submerged Fermentation Conditions of Nattokinase Production by Response Surface Methodology

作　　者：关志炜[1] 孙洪涛 

机构地区：[1]山东教育学院生物科学与技术系,山东济南250013 [2]山东福瑞达生物工程有限公司,山东济南250014

出　　处：《山东教育学院学报》2009年第3期65-69,共5页Journal of Shandong Education Institute

摘　　要：为了提高纳豆激酶的酶活力,本研究借助Minitab软件,采用Plackett-Burman试验设计方法和响应面分析方法对保存的枯草芽孢杆菌纳豆亚种(Bacillus subtlis natto,简称纳豆菌)-NT207进行了液体发酵条件的优化。首先通过Plackett-Burman方法对11个相关影响因素的效应进行评价,并筛选出有显著正效应的木糖浓度和有显著负效应的接种量、K2HPO4浓度等三个因素,然后利用响应面分析方法确定了上述三个因素的最佳工艺参数。实验结果表明,在优化后的发酵条件下,纳豆激酶的产量为1504.5IU/mL,比优化前提高了130.3%。In order to increase the nattokinase activity of Bacillus subtilis natto NT207, Plackett-Burman design and response surface methodology were utilized to optimize its submerged fermentation conditions by Minitab software. First, a Plackett-Burman design was used to evaluate the influence of the related factors. It showed that xylose influenced NK production positively while the pitching rate and K2 HPO4 concentration negatively. The other factors had no significant influence on NK production. And then, response surface methodology was used to calculate the parameters of the 3 factors above. The optimized conditions allowed the NK production to be increased to 1504.5IU/mL and more remarkable than that before optimized by 130.3%.

关 键 词：纳豆激酶 液体发酵 PLACKETT-BURMAN设计 响应面法 

分 类 号：TS201.3[轻工技术与工程—食品科学]