纤维素酶热稳定机制的计算分析  

Computational Analysis of Thermostability of Cellulase

作　　者：曾沛斌[1] 潘力[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代食品科技》2009年第6期592-595,607,共5页Modern Food Science and Technology

基　　金：广东省科技攻关项目:2007A010900001

摘　　要：对水解糖苷酶第45家族的纤维素酶系进行计算分析,计算结果表明,与此家族酶系的热稳定性呈负相关的氨基酸对为:丙氨酸-谷氨酸(AE)、苯丙氨酸-赖氨酸(FK)和丙氨酸-异亮氨酸(AI);呈正相关的氨基酸对:甲硫氨酸-脯氨酸(MP)和甲硫氨酸-甘氨酸(MG),并引入氨基酸热稳定常量计算验证其相关性,发现呈负相关的氨基酸对均为显著相关,而呈正相关的氨基酸对显著性不明显。这个结果可以为此家族纤维素酶的基因工程改造提供一定参考。Two bioinformatic methods were combined to analyze the significant dipeptides responsible for optimum temperature of endoglucanases in Glycoside hydrolase in GHF 45. It was found that the negative amino acid pairs were Alanine-Glutamic acid, Phenylalanine-Lysine and Alanine-Isoleucine, which had significant physical-chemical, energetic and conformational properties that contribute to the thermostability of ceUulase in this family, while the positive dipeptides were Methionine-Proline, and Methionine-Glycine, whose corresponding properties were not notable. The result was useful for cellulase engineering for high temperature activity.

关 键 词：计算分析 氨基酸对 最适作用温度 纤维素酶 热稳定性 

分 类 号：TQ352[化学工程]