普洱茶与绿茶提取物体外抑菌效应的研究  

in vitro Antibacterial Activity of Pu'er Tea and Green Tea Extracts

作　　者：胡永金[1] 乔金玲[2] 葛长荣[2] 

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《食品科学》2009年第13期56-59,共4页Food Science

基　　金：云南省自然科学基金项目(2005C0040M)

摘　　要：采用滤纸片扩散法分别研究不同浓度的普洱茶和绿茶提取物对李斯特氏菌(L.monocytogenes)、猪粪链球菌(S.faecalis)、大肠杆菌(E.coli)、鼠伤寒沙门氏菌(S.typhimurium)、金黄色葡萄球菌(S.aureus)和炭疽杆菌(B.anthracis)6株食源性微生物和致病菌的抑制效果及其最小抑菌浓度(MIC)。结果表明:普洱茶提取物在1%～7%(m/V)范围内对李斯特氏菌(L.monocytogenes)有较强的抑制作用,其MIC为0.07mg/ml,在5%～7%范围内对所有供试菌种均有抑制作用,对炭疽杆菌的抑制效果最好。绿茶提取物在1%～2%(m/V)范围内仅对李斯特氏菌(L.monocytogenes)有抑制作用,其MIC为0.09mg/ml,在3%时仅对炭疽杆菌(B.anthracis)无抑制作用,在4%～7%范围内对所有供试菌种都有抑制作用,对猪粪链球菌抑制效果最好。The filter paper disc diffusion method was employed to determine the in vitro antibacterial activities of water extracts from Pu＇er tea and green tea against 6 foodborne pathogenic bacterial species including L. monocytogenes, S. faecalis, E. coli, S. typhimurium, S. aureus and B. anthraci and the minimal inhibition concentrations （MICs）. The results showed that the extract from Pu＇er tea displayed higher antibacterial activity against L. monocytogenes within the concentration range of 1% -- 7% （m/V） and the MIC was 0.07 mg/ml, and this extract presented inhibitory activity against all the 6 bacterial species within the concentration range of 5% -- 7%, especially against B. anthraci. However, the green tea extract only demonstrated inhibitory activity within the range of 1%--2% against L monocytogenes, with the MIC of 0.09 mg/ml, but at the concentration of 3% this extract exhibited inhibitory activity against other 5 bacterial species except B. anthraci, while it exhibited inhibitory activity against all the 6 bacterial species within the range of 4% -- 7%, having the highest activity against S. faecalis.

关 键 词：普洱茶 绿茶 致病菌 体外抑菌活性 最小抑菌浓度(MIC) 

分 类 号：TS202.3[轻工技术与工程—食品科学]