利用响应面分析法优化高山被孢霉ME-1生产花生四烯酸培养基成分研究(英文)  

Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology

作　　者：彭超[1] 黄和[1,2] 金明杰 肖爱华[2] 于文涛[1,2] 刘欣[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009 [2]南京工业大学江苏省工业生物技术创新中心,江苏南京211816

出　　处：《食品科学》2009年第13期205-211,共7页Food Science

基　　金：国家"973"重点基础研究发展计划项目(2007CB707805);国家"863"计划项目(2006AA02Z240);国家自然科学基金项目(20576054)

摘　　要：在利用高山被孢霉ME-1生产花生四烯酸(ARA)过程中,采用响应面分析法,对摇瓶中的培养基成分进行优化。建立了两个标准的多项式模型:在长达6.5d发酵过程中,当葡萄糖、酵母膏、KH2PO4和NaNO3浓度分别为90.16、12.50、3.80和3.54g/L时,生物量将到最大,约36.86g/L;当葡萄糖、酵母膏、KH2PO4和NaNO3浓度分别为103.16、11.66、3.80和3.43g/L时,AA产量将到最大,约9.65g/L。预测值通过实验得到了充分的证实,预测值和实验结果相关性很好。发酵罐实验结果表明,在高山被孢霉ME-1大规模发酵生产过程中,对培养基进行优化,将同时引起生物量(发酵5d,约34.21±1.01g/L)和AA产量(发酵6d,约9.86±0.45g/L)的增加。The media for biomass and arachidonic acid （ARA） production by Mortierella alpina ME-1 were optimized in shake flask cultures using response surface methodology （RSM）. Two imperial polynomial models were developed, and tae maximum biomass yield of 36.86 g/L that appeared at glucose, yeast extract （YE）, KH2O4 and NaNO3 of 90.16, 12.50, 3.80 and 3.54 g/L, respectively, while a maximum ARA yield of 9.65 g/L that appeared at glucose, YE, KH2PO4 and NaNO3 of 103.16, 11.66, 3.80 and 3.43 g/L, respectively, in 6.5-day fermentation were predicted. Verified by validation experiments showed excellent correlations between their predicted and measured values. The results of bioreactor fermentation also illustrated that the optimized culture medium enhanced both biomass （34.21 ± 1.01 g/L in 5-d fermentation） and ARA （9.86 ± 0.45 g/L in 6-d fermentation） production by M. alpine ME-1 in a large-scale fermentation process.

关 键 词：花生四烯酸 高山被孢霉 优化 响应面法 发酵 油脂 

分 类 号：TS201.3[轻工技术与工程—食品科学]