大鼠前脂肪细胞的培养及酪蛋白糖巨肽对其增殖的影响  

Cultivation of Rat Preadipocytes and Effects of Glycomacropeptide on Its Proliferation

作　　者：徐世平[1] 毛学英[1] 任发政[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2009年第13期253-255,共3页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD04A06);国家"863"计划项目(2008AA10Z320)

摘　　要：采用酶消化法从SD大鼠脂肪垫中分离得到前脂肪细胞。对分离的前脂肪细胞进行形态学观察,分析生长曲线。对前脂肪细胞进行诱导分化,并用油红O染色鉴定前脂肪细胞。结果表明:该方法能够得到增殖快、成脂分化率高的前脂肪细胞。还从乳清浓缩蛋白中分离酪蛋白糖巨肽(CGMP),初步研究其对体外前脂肪细胞的影响,发现CGMP能抑制前脂肪细胞的增殖。Preadipocytes were isolated from adipose tissue of SD rats by enzyme digestion, and their cellular morphology and growth curve were analyzed. Furthermore, preadipocytes were induced to differentiation and identified by Oil Red O staining. The results showed that this method can obtain preadipocytes with rapid proliferation and high rate of inducible adipogenic differentiation. Besides, this study also focused on the effects of glycomacropeptide （GMP） isolated from whey protein concentrate on the proliferation of preadipocytes. It was found that the GMP can inhibit the proliferation of preadipocytes.

关 键 词：前脂肪细胞 原代培养 鉴定 酪蛋白糖巨肽 

分 类 号：Q253[生物学—细胞生物学]