稻草秸秆的预处理及发酵乙醇的试验研究  

Pretreatment for Ethanol Fermentation of Rice Straw

作　　者：叶生梅[1] 许盼吉[1] 汪江林[1] 

机构地区：[1]安徽工程科技学院生物化学工程系,芜湖241000

出　　处：《中国粮油学报》2009年第6期133-136,共4页Journal of the Chinese Cereals and Oils Association

基　　金：安徽省教育厅自然科学基金资助项目(2003kj039)

摘　　要：通过不同的化学试剂与“汽爆”组合处理稻草秸秆的试验研究，确定了有效的预处理条件：稻草秸秆与3％氢氧化钠溶液（固液比W／V为1：5）混匀，置0．15MPa（126～128℃）保温5min，放气。此预处理能使秸秆中木质素去除75．58％；秸秆酶解糖化率（2％底物，0．2％纤维素酶，pH4．8，0．2mol／L醋酸-醋酸钠缓冲溶液，46℃水解48h）达82．78％；通过酿酒酵母将秸秆纤维素酶解糖化液发酵转化为乙醇，产乙醇浓度达5．0％（V／V）。An effective pretreatment advantageous for the ethanol fermentation of rice straw was studied. The determined pretreatment procedure is as follows： blending rice straw and 3 % NaOH in solid/liquid ratio of 1：5 （w/v）, insulating for 5 min at 0.15 MPa （126 - 128℃）, deflating. Results show： Through this pretreatment 75.58% lignin in the straw can be removed. The enzymatic sacchafifieation rate of the pretreated rice straw reaches 82.78 % at the following condition： substrate 2%, cellulase 0.2%, pH 4.8, 0.2 mol/L HAc - NaAc buffer solution, temperature 46℃, hydrolysis time 48 h. The brew yeast Saecharomyces eerevisiae can grow on the saeeharified hquid and convert the sugar into ethanol, producing ethanol liquid in concentration 5.0% （V/V）.

关 键 词：稻草秸秆 预处理 纤维素 纤维素酶 发酵 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]