不同柑橘品种生理落果中橙皮苷和辛弗林含量测定  

Determination of Hesperidin and Synephrine in Physiologically Fallen Fruits of Various Citrus Species

作　　者：毛华荣[1] 傅虹飞[1] 王鲁峰[1] 吴鹏[1] 卢琪[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2009年第14期223-226,共4页Food Science

摘　　要：测定不同柑橘品种两次生理落果中橙皮苷和辛弗林的含量,比较原料采集后不同干燥方式对含量的影响。采用高效液相色谱法,色谱柱HypercilODS2(250mm×4.6mm,5μm),流动相为水-乙腈-四氢呋喃-乙酸(80:16:3:1,V/V),波长280nm测定橙皮苷的含量;流动相为乙腈-水-磷酸(32:68:0.2,V/V),内含0.2gSDS/100ml,波长225nm测定辛弗林的含量。结果表明:7个品种经80℃鼓风干燥后测得橙皮苷含量均高于其他处理(自然风干,40、60℃鼓风干燥),而60℃处理后的生理落果测得的含量辛弗林含量均大于其他处理。不同柑橘品种间两次生理落果的橙皮苷和辛弗林含量差异显著,各品种第二次生理落果除琯溪蜜柚和酸橙橙皮苷含量均高于第一次,其中琯溪蜜柚第一次落果橙皮苷含量最高,达(199.28±0.47)μg/mg,各品种第二次生理落果辛弗林含量均较第一次有所降低,尾张第一次落果辛弗林含量最高,达(20.50±0.89)μg/mg。Hesperidin is one of the flavonoids existing in citrus fruit peel or physiologically fallen fruit before maturity, especially in the latter with over 3% content. Synephrine, an alkaloid, is mainly contained in immature citrus fruit as well, and the more mature the fruit becomes, the lower its content is. The physiologically fallen fruits of 7 citrus species were collected at two periods of pre-harvest fruit drop and dried by an up-draught drying fan or in natural air. These samples were analyzed for their hesperidin and synephrine contents by HPLC. The hesperidin analysis was carried out on a Hypercil ODS2 column （250 mm × 4.6 mm, 5 μm） using a mobile phase composed of water, acetonitrile, THF and acetic acid （80：16：3：1, V/V） with UV detection set at 280 nm; the mobile phase （containing 0.2 g SDS/100 ml） for analyzing synephrine was an acetonitrile-water-phosphoric acid （32：68：0.2, V/V） system, and the detection wavelength was set at 225 nm. The results indicated that the contents of hesperidin in all the citrus species treated with 80 ℃ hot air were higher than those treated by natural air, 40 ℃ hot air, or 60 ℃ hot air. The contents of synephrine in all citrus species treated by 60 ℃ hot air were higher than those with other treatments. Moreover, the contents of hesperidin and synephrine were different greatly among different citrus species and between the periods of preharvest fruit drop. The hesperidin content in citrus fruits at the second period of physiologic drop was higher than that at the first period except for Guanxi honey pomelo and sour orange. The highest content of hesperidin was observed in Guanxi honey pomelo at the first period, reaching （199.28±0.7） μg/mg. While the synephrine content in the fallen fruits of all the citrus species at the first period were higher than that at the second period, especially satsuma mandarin fruit （Citrus unshiu Mareovitch, cv. Owari） had the maximum synephrine content of （20.50 ±0.89） μ g/mg among all the specie

关 键 词：柑橘 生理落果 橙皮苷 辛弗林 高效液相色谱法 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]