黑曲霉α-鼠李糖苷酶高产菌株的选育  

Screening of α-Rhamnosidase High-yield Strain from Aspergillus niger

作　　者：陈华根[1] 倪辉[1] 李利君[1] 肖安风[1] 苏文金[1] 蔡慧农[1] 

机构地区：[1]集美大学生物工程学院发酵工程研究室,福建厦门361021

出　　处：《微生物学通报》2009年第7期1008-1012,共5页Microbiology China

基　　金：福建省自然科学基金项目(No.B0610030);福建省青年人才创新基金(No.2007F3073);厦门市科技计划项目(No.3502E-2006-3016)

摘　　要：本文首次报道利用Davis方法制备的透明圈法筛选α-鼠李糖苷酶高产菌株。用甲基磺酸乙酯对出芽8h的黑曲霉孢子进行诱变处理,用透明圈法初筛出的菌株中,产量提高40%以上的突变菌株占11%;用摇瓶培养对初筛出的菌株进行两轮复筛α-鼠李糖苷酶高产突变株T-226,摇瓶培养α-鼠李糖苷酶活达373.4U/mL,比出发菌株提高了22.7%。对该高产突变株进行5L罐发酵实验,发酵84h测得α-鼠李糖苷酶活为631.9U/mL。用新建立的方法选育高产α-鼠李糖苷酶的高产菌株,不仅具有较高的筛选效率,还具有良好的准确性。In this study, plate transparent circle by Davis method was introduce firstly screening α-Rhamnosidase high-yield strain. The spore-sprouted Aspergillus niger 8-hour were mutagenized by ethyl methane sulphonate and pre-screened via transparent circle. 11% mutants yield 40% higher of α-rhamnosidase than the original strain. A high-yield strain, T-226 with the highest α-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yield mutants after rescreened by shake flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate α-rhamnosidase high-yield mutant of A. niger.

关 键 词：黑曲霉 α-鼠李糖苷酶 诱变育种 透明圈 发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]