PVA-SA-活性炭共聚物固定脂肪酶的研究  

Study of the Lipase Immobilized on PVA-Alginate-Active Carbon

作　　者：孙凤麟[1] 孙玉梅[1] 曹芳[1] 王珊[1] 

机构地区：[1]大连工业大学生物与食品工程学院,大连116034

出　　处：《食品与生物技术学报》2009年第4期479-482,共4页Journal of Food Science and Biotechnology

基　　金：辽宁省教育厅2006年创新团队项目(2006T030)

摘　　要：以聚乙烯醇(PVA)-海藻酸钠(SA)-活性炭共聚物为载体,对脂肪酶进行固定化。研究了活性炭浓度、酶初始浓度、缓冲液pH值、吸附时间及温度对固定化酶的活性及蛋白载量的影响。结果表明:在聚乙烯醇(PVA)-海藻酸钠凝胶中加入1 g/dL活性炭制成复合凝胶球,在25℃、pH值5.5的条件下对30 mg/mL的酶液吸附12 h,所得吸附固定化脂肪酶的活性较好。吸附固定的脂肪酶较游离酶的最适作用温度升高10℃,最适作用pH范围变宽,且向碱方向偏移0.2个单位。In this manuscript, the immobilized conditions, such as active carbon concentration in the gel, enzyme concentration, pH, adsorption time and temperature, of lipase to PVA--alginate --active carbon were carefully investigated. It was found that a highest lipase activity was achieved at the condition of 30 mg/ml attached to 1 % PVA-- alginate gel pellet when temperature is 25℃ and pH is 5. 5. Compared with that of free lipase, the optimal reaction temperature increased by 10℃ and pH range extended to 7.2.

关 键 词：脂肪酶 聚乙烯醇 海藻酸钠 活性炭 固定化 

分 类 号：Q814[生物学—生物工程]