盐酸羟胺和紫外线复合诱变选育纳豆激酶高产菌株  

Research on the Breeding of Bacillus subtilis Natto Highly Producing Nattokinase by the Compound Mutation of Hydroxylamine Hydrochloride and UV Radiation

作　　者：关志炜[1] 李志香[1] 孙洪涛 

机构地区：[1]山东教育学院生物科学与技术系 [2]山东福瑞达生物工程有限公司,山东济南250014

出　　处：《食品与发酵工业》2009年第6期71-74,共4页Food and Fermentation Industries

摘　　要：以从日本纳豆中分离出的1株枯草芽孢杆菌纳豆亚种(Bacillus subtlis natto,简称纳豆菌)NT-6为出发菌株,通过盐酸羟胺和紫外线复合诱变处理,采用利福平抗性平板筛选和发酵筛选相结合的方法,获得了1株突变株NT207,其产酶能力比诱变前提高了68%,在未经优化的液态发酵条件下,纳豆激酶的酶活力达到了648.72IU/mL。Bacillus subtilis natto NT-6 strain was isolated from a kind of Japanese functional food. In order to enhance the nattokinase-producing ability of NT-6, the compound mutation of hydroxylamine hydrochloride and UV radiation was performed. After the compound mutation, the mutant strain NT207 was obtained by the screening of anti-rifampin plate and then liquid fermentation. NT207 ＇ s ability of producing nattokinase was more remarkable than the strain NT-6 by 68%. The activity of nattokinase produced by the strain NT207 in the condition of the liquid fermentation medium that hasn＇ t been optimized can reach to 648.72 IU/mL.

关 键 词：纳豆激酶 菌种选育 酶活力 复合诱变 

分 类 号：TQ925[轻工技术与工程—发酵工程]