油松花粉多糖分离纯化及结构分析的初步研究  

Separation,Purification and Structural Analysis of Polysaccharide from Chinese Pine Pollen

作　　者：范三红[1] 刘艳荣[1] 杨民乐[1] 周立波[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006

出　　处：《食品科学》2009年第15期127-129,共3页Food Science

摘　　要：油松花粉经热水提取,Sevag法除蛋白,乙醇沉淀得多糖。采用Sephadex G-100柱层析对多糖进行初步分离纯化,用红外光谱、紫外-可见光谱、气相色谱分析对花粉多糖的结构进行初步研究。结果表明:纯化的油松花粉多糖为杂多糖,至少由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖及半乳糖6种单糖组成。另外,GC图谱显示油松花粉多糖还含有一个未知的单糖,峰面积非常小,含量比较低。UV检测显示油松花粉多糖在波长260nm与280nm处未见有核酸和蛋白特征吸收峰,经IR检测证实油松花粉多糖具有多糖类物质的特征吸收峰。A polysaccharide from Chinese pine pollen was obtained through hot water extraction, ethanol precipitation, Sevag deproteinization and Sephadex G-100 chromatography and was analyzed structurally by IR spectroscopy, UV-vis spectroscopy and gas chromatography （GC）. The polysaccharide was highly heterogeneous and was mainly composed of rhamnose arabinose, xylose,marmose,glucose and galactose. Besides, GC analysis revealed that the polysaccharide contained an unknown monosaccharide with a small peak area suggesting low content. No characteristic absorption of nucleic acid or protein in the polysaccharide was observed in its UV-vis spectrum and it exhibited characteristic IR absorption of polysaccharide.

关 键 词：油松花粉 多糖 分离纯化 结构分析 

分 类 号：Q539[生物学—生物化学]