动物双歧杆菌01耐硒性能及富硒条件的研究  

Selenium Tolerance and Enrichment in Bifidobacterium animalis 01

作　　者：靳志强[1] 张博文[2] 李平兰[2] 

机构地区：[1]长治学院生化系,山西长治046011 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2009年第15期184-187,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD05A03)

摘　　要：对动物双歧杆菌01的耐硒性能和最适富硒条件进行初步研究。结果显示:当MRS培养基中硒(Ⅳ)含量为12μg/ml时,即为动物双歧杆菌01的最大耐硒量;动物双歧杆菌01富硒最适培养条件为:培养基硒(Ⅳ)浓度8μg/ml,接种量5%,培养6h时加硒,培养时间为48h;在最适培养条件下进行培养后,其菌体硒含量高达(898±74)μg/g,硒转化率为(28.1±2.4)%。The selenium tolerance of Bifidobacterium animalis 01 isolated from feces of long-life persons living in Bama area of Guangxi in China was investigated and the conditions for selenium enrichment in this strain were optimized by single-factor and orthogonal array methods. The maximum concentration of selenium in the culture medium was 12μg/ml for Bifidobacterium animalis 01. The optimal conditions for selenium enrichment in Bifidobacterium animalis 01 were determined as follows： Se（Ⅳ） concentration 8μg/ml, inoculation amount 5%, adding selenium to the medium at the 6^th of culture, and culture time 48 h. Under these conditions, the selenium content in Bifidobacterium animalis 01reached （898 ± 74） μg/g and the conversion rate of selenium was （28.1 ± 2.4）%.

关 键 词：双歧杆菌 耐硒 富硒 

分 类 号：Q935[生物学—微生物学]