大叶龙茶解剖学、肌动蛋白基因及分子标记的研究  

Study on Anatomy, Actin Gene and Molecular Marker of Tea Cultivar Dayelong

作　　者：徐玲玲[1] 张美云[1] 李同建[1] 秦红霞[1] 赵中伟[1] 樊启水 廖亮[1] 张大明[1] 

机构地区：[1]九江学院生命科学学院,江西九江332000 [2]江西修水茶叶研究所,江西修水332400

出　　处：《茶叶科学》2009年第4期263-270,共8页Journal of Tea Science

基　　金：江西省教育厅科技项目;江西省九江市科技项目

摘　　要：大叶龙茶是来源于江西修水茶科所宁州群体茶园中一株自然突变体形成的大叶并无蕾无性繁殖新品种。对大叶龙茶及其母株的成熟叶片和不同时期的芽进行石蜡切片研究表明,大叶龙茶叶片栅栏组织细胞较长、海绵组织层数增加;大叶龙茶无蕾的原因是由于所有的芽始终保持营养芽的状态所致。首次克隆了茶树3个肌动蛋白基因片段(CS-ACT1,CS-ACT2,CS-ACT3),且都编码225个氨基酸,大叶龙茶与母株及500个BlastX分析获得的肌动蛋白序列在保守结构域F、G、H区有相应的四个氨基酸不同。对大叶龙茶及其母株进行AFLP分析表明,大叶龙茶总带数比母株少,并出现一部分特有带;利用EST-SSR分子标记技术对大叶龙茶及其它常见品种进行研究,表明大叶龙茶等4个修水品种与龙井43有相似的遗传基础。Tea Cultivar Dayelong is a vegetative propagation cultivar of large-leaf and budless mutated from a stock plant of Ningzhou population. The paraffin section of mature leaves and buds of different period from Dayelong and its stock plant was investigated. Results showed that the palisade cell was rather long, the layers of spongy cell increased. Dayelong do not have a flower bud is due to all the buds always maintain the situation of vegetative bud. Three actin gene fragments （CS-ACT1, CS-ACT2, CS-ACT3） of tea plant were cloned for the first time. They encoding 225 amino acids. Dayelong, its stock plant and other 500 Actin squences obtained by BlastX analysis showed four amino acids of Dayelong are different from those of corresponding sites on conservative domains F, G and H. The Dayelong and its stock plant for AFLP analysis showed that the band numbers of Dayelong is fewer than the stock plant and has its own specific band. EST-SSR molecular markers technique was used to analyze Dayelong and other cultivars, the results showed that four cultivars of Xiushui including the Dayelong are similar to Longjing 43 in genetic basis.

关 键 词：大叶龙茶 解剖学 肌动蛋白基因 AFLP EST-SSR 

分 类 号：S571.1[农业科学—茶叶生产加工] Q942[农业科学—作物学]