乳链菌肽高产菌株的推理选育  

Rational screening of high producing nisin in strain

作　　者：姜丽艳[1] 闫国栋[1] 张宏宇[1] 兰鹏飞[1] 刘海龙[1] 孟庆繁[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《食品与机械》2009年第4期38-40,共3页Food and Machinery

基　　金：2007年"吉林大学大学生创新性试验计划"资助项目(编号:JDCX2008026)

摘　　要：采用硫酸二乙酯的化学诱变方法,通过对突变株的推理选育筛选得一株乳链菌肽抗性菌株L-D-2,效价最高达到2 570 IU/mL,比出发菌株提高了2.13倍。该菌株传代的稳定性试验表明:L-D-2有较稳定的产乳链菌肽的遗传特性。推理选育乳链菌肽高产菌株的方法快速、有效,可以进一步推广。Applied the way of DES mutation and filter mutation stain through rational screening. The mutant L-D-2 was obtained, which has a productivity with 2570 IU/mL and was 2. 13 times higher than the original strain. The experiment results of L-D-2 continuous generations showed that the strain had higher and stable genetic characteristic. The way of rational screening high producing Nisin strain is clipping and effective,which can be more extended.

关 键 词：乳链菌肽 硫酸二乙酯 诱变 推理选育 

分 类 号：TS201.2[轻工技术与工程—食品科学]