紫外-LiCl复合诱变选育高产糖化酶菌株  

Breeding of High-yield Glucoamylase Strains by UV-LiCI Mutagensis

作　　者：董会平[1] 夏帆[1] 张华山[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068

出　　处：《酿酒科技》2009年第8期33-34,37,共3页Liquor-Making Science & Technology

基　　金：2007年度湖北省农业科技成果转化资金计划项目

摘　　要：对糖化酵母As2.1548的紫外-LiCl复合诱变条件进行研究。在紫外照射时间90s、LiCl用量0.5%时,其正突变率达到21.8%。成功选育出一株遗传性能稳定的高产糖化酶菌株A-U-90-8,其产糖化酶活力为324U/g,比出发菌株提高了48.6%。The UV-LiCI mutagensis conditions of saccharifying yeast As2.1548 were investigated. As UV irradiation time was 90 s and LiCI dosage was 0.5 %, the positive mutation rate could reach up to 21.8 %. Then a genetically-stable high-yield glucoamylase strain A-U-90-8 was obtained successfully with its glucoamylase activity as 324 U/g （increasing by 48.6 % than that of the starting strain）.

关 键 词：微生物 糖化酵母 糖化酶活力 紫外-LiCl复合诱变 

分 类 号：TS201.25[轻工技术与工程—食品科学]