荞麦谷蛋白酶法水解制备抗氧化活性肽  

Study on Preparation of Antioxidant Peptides from Buckwheat Glutelin by Enzyme Hydrolysis

作　　者：付媛[1,2] 张美莉[1] 王艳菊 侯文娟[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]内蒙古农业大学理学院,内蒙古呼和浩特010018 [3]内蒙古兴安盟农业技术推广站,内蒙古乌兰浩特137400

出　　处：《食品与发酵工业》2009年第7期82-86,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(No.30760151)

摘　　要：以水解度和对O2-.清除率为指标,采用正交试验对碱性蛋白酶酶解荞麦谷蛋白工艺进行探索,实验结果,酶水解最佳工艺为:温度45℃,pH值10,底物浓度30g/L,酶底比为10%(质量分数),水解时间为2h。在此条件下,水解度可达20.72%,对O2-.清除率达45.27%。对酶解产物进行SephadexG-25柱层析分离,得到组分Ⅰ和组分Ⅱ。其分子质量范围分别为20.89-95.5ku,2138-4570u。组分Ⅰ和组分Ⅱ对O2-.、.OH、DPPH.三种自由基均具有清除能力,且组分Ⅰ的清除能力最强。组分Ⅰ对3种自由基清除能力表现为.OH>DPPH.>O2-.,其IC50分别为:0.21mg/mL,0.24mg/mL和0.31mg/mL。Taking degree of hydrolysis （DH%） and scavenging activity of O2 - · as criterion, the alkaline protease hydrolysis of buckwheat glutelin was studied by orthogonal test. The obtained optimum hydrolysis parameters were as follows： temperature 45 ℃ , pH 10, concentration of substrate 30 g/L, dose of enzyme 10% , time 2 h. With these parameters, the DH% can be reached 20.72% ,the ratio of scavenging O2^ - can be reached 45.27%. The hydrolyzate was separated by Sephadex G -25 gel filtration chromatography, then we obtained composition Ⅰand Ⅱ. Their molecular weights were 20. 89 -95.5 ku, and 2 138 -4 570 u. The abilities for scavenging O2 - · OH and DPPH · of composition Ⅰ were better than composition Ⅱ. The comparison of composition I for scavenging the three free radicals were · OH 〉 DPPH · 〉 O2- ·,the IC50 were 0.21 mg/mL,0.24 mg/mL,0.31 mg/mL.

关 键 词：荞麦 谷蛋白 活性肽 蛋白酶 抗氧化 自由基 

分 类 号：TQ464.7[化学工程—制药化工]