反相高效液相色谱法同时测定10种食品添加剂  

Simultaneous determination of ten food additives by reversed-phase high performance liquid chromatography

作　　者：阿布都外力·吐尼亚孜[1] 杨涛 鹿毅 史瑞芬[1] 段晋峰[1] 王吉德[1] 

机构地区：[1]新疆大学化学化工学院,乌鲁木齐830046 [2]新疆产品质量监督检验研究院,乌鲁木齐830004

出　　处：《食品科技》2009年第8期275-279,共5页Food Science and Technology

摘　　要：采用反相高效液相色谱法，同时测定食品中常见的10种食品添加剂：苯甲酸、山梨酸、对羟基苯甲酸甲酯（尼泊金甲酯）、糖精钠、安赛蜜、柠檬黄、日落黄、胭脂红、苋菜红、亮蓝。实验采用Symmetry C18柱（4．6mm×150mm，5μm，Waters）为分离柱，以20mmol／L乙酸铵（pH6．8）-甲醇为流动相，用紫外检测器在234nm下进行检测，整个分析过程在17min内完成。其平均加标回收率为94．14％～99．94％，相对标准偏差小于4％。该方法结果准确，适用于食品常规质量检测。A novel method is proposed for the simultaneous separation and determination of ten food additives （benzoic acid, sorbic acid, methyl p-hydroxybenzoate, saccharin sodium, acesulfame potassium, tartrazine, sunset yellow, ponceau 4R, amaranth, brilliant blue） by reversed-phase high performance liquid chromatography. The separation was achieved within 17 min by using a Symmetry reverse phase C18 colum （150 mm ×4.6 mm, 5μm, Waters Inc） with 20 mmol/L NH4Ac （pH6.8）-methanol as mobile phase and lastly detected at 234 nm through a UV detector. The average recoveries for real samples ranged from 94.14% to 99.94%. The relative standard deviation was less than 4%, This method is accurate and applicable to conventional food quality testing.

关 键 词：食品 食品添加剂 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]