青霉菌产植酸酶液体发酵条件研究  

Studies on the Culture Conditions of a Strain of Producing-phytase Penicillium sp.

作　　者：褚西宁[1,2,3] 赵美蓉 裴武红[1,2,3] 

机构地区：[1]山西大学生命科学系 [2]军事医学科学院基础医学研究所 [3]山西长治医学院微生物学教研室

出　　处：《山西大学学报（自然科学版）》1998年第3期263-267,共5页Journal of Shanxi University(Natural Science Edition)

摘　　要：从油脂废料中分离到一株产植酸酶的青霉菌株。研究了不同碳源，氮源对青霉菌液体发酵产植酸酶的影响。结果最佳碳源为蔗糖，玉米淀粉。最佳氮源为硝酸铵、蛋白胨。通过正交试验获得适于产植酸酶的液体培养基的配方为：玉米淀粉２％，蔗糖１．５％，蛋白胨１％，硝酸铵０．２％以及少许无机盐。在此基础上，对其他影响植酸酶产生的因素也作了研究。The culture conditions of a strain of producing-phytase penicillium separated from the oil waste were reported.The effect of the various carbon and nitrogen sources on this strain was studied.The results indicated that the optimal carbon and nitrogen compounds for this strain were sucrose,corn starch and ammonia nitrate,peptone respectively.The best media obtained from the orthogonal test in this culture conditions are as follows:corn starch 2%,sucrose 1.5%,peptone 1%,ammonia nitrate 0.2% and a small amounts of mineral salts.On the basis of the experimants,the other factors such as pH etc.were also mentioned.

关 键 词：青霉菌 植酸酶 发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]