超高效液相色谱法测定皮革样品中富马酸二甲酯  

Determination of dimethy fumarate residue in leathers by ultra performance liquid chromatography

作　　者：冯楠[1] 路勇[1] 吴颖[2] 张卫民[1] 姜洁[1] 谢文东[1] 刘丽娟[2] 

机构地区：[1]北京市食品安全监控中心,北京100041 [2]北京市产品质量监督检验所,北京100029

出　　处：《中国卫生检验杂志》2009年第8期1801-1803,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立皮革样品中富马酸二甲酯残留量的UPLC测定方法和样品前处理方法。方法：样品经乙腈提取后,ENVI-Carb固相萃取柱和中性氧化铝固相萃取柱净化,进行UPLC定量分析。试验采用BEH C18色谱柱,以水和乙腈为流动相。结果：方法测定低限为0.1 mg/kg,在0.1-5 mg/kg添加水平范围内,富马酸二甲酯回收率为71.9%-80.1%,相对标准偏差为2.5%-4.6%。结论：方法准确度和稳定性较好,操作简单快捷,样品净化效果好,测定低限能够满足限量要求。Objective： A UPLC method for the determination of dimethy fumarate residue in leathers has been develoed.Methods：Dimethy furmarate was extracted from sample with acetonitrile and cleaned up with ENVI-Carb SPE cartridge and ALUMINA N SPE cartridge;The analyte was determinated by UPLC for the quantitative determination,and quantified by the external standard curve.The separation was performed on a UPLC BEH C18 column with a system of water-acetonitrile as mobile phase at a flow rate of 0.3 ml/min.Results：The limit of determination of this method was 0.1 mg/kg.The detection limits of 0.15 mg/kg proved to be much better than the previously reported ones.Average recoveries of Dimethy fumarate ranged from 71.9% to 80.1%,with relative standard deviations between 2.5%～4.6%.Conclusion：The method is simple,accurate and suitable for the identification and quantification of dimethy fumarate residue in leathers.

关 键 词：超高效液相色谱 皮革 富马酸二甲酯 残留 测定 

分 类 号：R917[医药卫生—药物分析学]