快速分离液相色谱/串联四级杆电喷雾质谱法同时测定食品中多种人工合成甜味剂  

Simultaneous determination of various kinds of synthetic sweeteners in foods by RPLC/MS/MS

作　　者：李传慧[1] 李淑娟[1] 安娟[1] 李建中[1] 

机构地区：[1]中国检验检疫科学研究院,食品安全研究所,北京100123

出　　处：《食品工业科技》2009年第8期319-320,358,共3页Science and Technology of Food Industry

基　　金：国家质检总局项目基金资助课题(2007IK263)

摘　　要：建立了同时检测食品中糖精钠(SA)、安赛蜜(AK)、甜蜜素(SC)、阿斯巴甜(ASP)、纽甜(NTM)5种人工合成甜味剂的RPLC/MS/MS分析方法。色谱分离采用ZOBAX SB-C18(2·1mm×50mm,1·8μm),以乙腈-水为流动相,ESI(-)电离模式下,采用多反应监测(MRM)进行监测,5种甜味剂的平均添加回收率在82·3%～104·3%之间,相对标准偏差(RSD)≤2·0%。本方法可用于食品中5种人工合成甜味剂的同时测定。A RPLC/MS/MS method was developed for the determination of five synthetic sweeteners, saccharin sodium（ SA）, acesulfame potassium （ AK）, sodium cyclamate （ SC ）, aspartame （ ASP ） and neotame （ NTM ） in foods.The RPLC separation was performed on a ZQBAX SB-C,8 （50mm × 2.1mm, 1.8μm） with a gradient elution program of acetonitrile and water as the mobile phase.The identification and quantification were done with mass spectrometry.The average recoveries of five synthetic sweeteners ranged from 82.3% to t04.3% with relative standard deviations below 2.0%.This method can be used for the simultaneous determination of these sweeteners.

关 键 词：人工合成甜味剂 快速高效液相色谱/串联质谱 测定 食品 

分 类 号：TS207.3[轻工技术与工程—食品科学]