磷脂酶固定化方法的研究  

Immobilization methods of phospholipase

作　　者：张智[1] 王瑾[1] 王腾宇[1] 于殿宇[1] 杨帆[2] 曹万新[2] 魏冰[2] 石珊珊[2] 杜宣利[2] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]国家粮食储备局西安油脂科学研究设计院,西安710082

出　　处：《中国油脂》2009年第9期35-38,共4页China Oils and Fats

基　　金：国家科技支撑计划课题"双低油菜制油新技术与系列产品开发及产业化示范"(2006BAD05A12)

摘　　要：分别采用海藻酸钠、海藻酸钠-壳聚糖和海藻酸钠-明胶固定化磷脂酶,研究发现固定化磷脂酶最适反应温度比游离酶提高10℃左右,反应适宜pH范围明显变宽。海藻酸钠-壳聚糖固定化磷脂酶的热稳定性最好,操作稳定性由强至弱为海藻酸钠-明胶固定化酶、海藻酸钠-壳聚糖固定化酶、海藻酸钠固定化酶,重复使用4次后酶相对活力分别为80%、80%和50%。固定磷脂酶的最佳载体为海藻酸钠-壳聚糖。Sodium alginate, sodium alginate- ehitosan and sodium alginate -gelatin were used to immobilize phospholipase respectively. The characteristics of the immobilized phospholipase were studied. The results showed that the reaction suitable pH range of immobilized enzyme was obviously broadened and the optimum reaction temperature imcreased about 10 ℃. The thermal stability of immobilized phospholipase by sodium alginate - chitosan was best. The operational stability of immobilized enzymes by the three immobilization vectors decreased in the order： sodium alginate -gelatin, sodium alginate -chitosan and sodium alginate, and the corresponding activity of the immobilized enzyme was 80% ,80% and 50% respectively after reused for four times. The sodium alginate - chitosan was the best vector to immobihze phospholipase.

关 键 词：海藻酸钠 壳聚糖 明胶 磷脂酶A1 固定化 

分 类 号：Q814[生物学—生物工程]