乳酸菌β-葡萄糖苷酶水解大豆异黄酮的研究  

Hydrolysis of soybean isoflavones by β-glucosidases from lactic acid bacterium

作　　者：沈玥[1] 张永忠[1,2] 孙艳梅[1,2] 

机构地区：[1]东北农业大学应用化学系,哈尔滨150030 [2]教育部大豆生物学重点实验室,哈尔滨150030

出　　处：《中国油脂》2009年第9期60-63,共4页China Oils and Fats

基　　金：黑龙江省"十一五"重大科技攻关项目(GA06B402-4);大豆生物学教育部重点实验室主任基金项目(SB06A02)

摘　　要：利用乳酸菌液态发酵得到一种高活性的大豆异黄酮水解酶——乳酸菌β-葡萄糖苷酶,通过单因素试验及正交试验优化了乳酸菌β-葡萄糖苷酶水解大豆异黄酮的条件。正交试验结果显示,当加酶量为25μg/mL,水解温度50℃,水解时间2.0 h,pH6.0时,黄豆苷水解率可达96.51%;在加酶量为25μg/mL,水解温度40℃,水解时间2.0 h,pH5.5时,染料木苷水解率为92.36%。A high active hydrolysis enzyme of β - glucosidases on soybean isoilavones was obtained by liquid fermentation with lactic acid bacterium. The hydrolysis conditions of soybean isoflavones by β - glucosidases were optimized by single factor and orthogonal tests. The optimal hydrolysis conditions of daidzin were as follows ： concentration of enzyme 25 μg/mL, temperature 50℃, time 2.0 h, pH 6.0. The optimal hydrolysis conditions of genistin were as follows ： concentration of enzyme 25 μg/mL, temperature 40 ℃, time 2.0 h, pH 5.5. Under the optimal conditions, the hydrolysis rate of daidzin and genistin reached 96.51% and 92.36% ,respectively.

关 键 词：乳酸菌 Β-葡萄糖苷酶 大豆异黄酮 水解 

分 类 号：TQ461[化学工程—制药化工]