Effects of Tetrazanbigen on the Protein Expression in Human Hepatocellular Carcinoma Cell Line QGY-7701  

作　　者：袁拥华 李伟 李龙江 杨晓兰 谷容 刘华波 黄开顺 余瑜 

机构地区：[1]Department of Medicinal Chemistry ,Research Laboratory of Pharmaceutical Chemistry and Biomaterials, Chongqing Engineering Research Center of Drugs, School of Pharmacy [2]Pharmacy of Children's Hospital, Chongqing Medical University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2009年第3期304-308,共5页华中科技大学学报（医学英德文版）

基　　金：supported by grants from National Natural Sciences Foundation of China (No. 30772595, No. 3037163 2 and No. 30171070);National Foundation for New Drug Research and Development of China (No. 96-501-5-6);Chongqing Municipal Sciences Technology Commission (Yu Ke Fa 20021425)

摘　　要：Tetrazanbigen （TNBG） is a novel synthetic antitumor drug with significant antitumor effects on common solid tumors in vitro and in vivo. It may lead to death of cancer cells through a tumor-associated lipoidosis mechanism, and result in lipid droplets （LDs） accumulation at the cytoplasm. In this study, the effects of TNBG on protein expression in human hepatocellular carcinoma cell line QGY-7701 were studied for elucidating its antitumor mechanism. The proteins extracted from TNBG-treated human hepatocellular carcinoma cell line QGY-7701 were analyzed and com- pared with control cells by two-dimensional gel electrophoresis. The differential proteins were identified by matrix-associated laser desorption ionization time-of-flight mass （MALDI-TOF-MS） spec- trometry. Two proteins of interest, the levels of which were significantly increased in TNBG-treated cells, were further characterized by Western blot analysis. The results showed a total of 846±23 spots in control cells and 853±30 spots in TNBG-treated cells. Twenty-six up-regulated or down-regulated proteins were found by analyzing differential proteomic 2-DE map. Eleven of them were identified by mass spectrometry. They were protein disulfide-isomerase precursor, 94 kD glucose-regulated protein, heat shock protein （HSP） 90-alpha, ATP-citrate lyase, HMG-CoA reductase, glucose-6-phosphate 1-dehydrogenase, very-long-chain specific acyl-CoA dehydrogenase, squalene synthetase, sterol regulatory element-binding protein 1, fructose-bisphosphate aldolase A, and peroxiredoxin-1. These up-regulated or down-regulated proteins are mostly related to lipid metabolism. The TNBG antitumor mechanism is probably to influence tumor lipid metabolism, resulting in accumulation of LDs in tumor cells.Tetrazanbigen （TNBG） is a novel synthetic antitumor drug with significant antitumor effects on common solid tumors in vitro and in vivo. It may lead to death of cancer cells through a tumor-associated lipoidosis mechanism, and result in lipid droplets （LDs） accumulation at the cytoplasm. In this study, the effects of TNBG on protein expression in human hepatocellular carcinoma cell line QGY-7701 were studied for elucidating its antitumor mechanism. The proteins extracted from TNBG-treated human hepatocellular carcinoma cell line QGY-7701 were analyzed and com- pared with control cells by two-dimensional gel electrophoresis. The differential proteins were identified by matrix-associated laser desorption ionization time-of-flight mass （MALDI-TOF-MS） spec- trometry. Two proteins of interest, the levels of which were significantly increased in TNBG-treated cells, were further characterized by Western blot analysis. The results showed a total of 846±23 spots in control cells and 853±30 spots in TNBG-treated cells. Twenty-six up-regulated or down-regulated proteins were found by analyzing differential proteomic 2-DE map. Eleven of them were identified by mass spectrometry. They were protein disulfide-isomerase precursor, 94 kD glucose-regulated protein, heat shock protein （HSP） 90-alpha, ATP-citrate lyase, HMG-CoA reductase, glucose-6-phosphate 1-dehydrogenase, very-long-chain specific acyl-CoA dehydrogenase, squalene synthetase, sterol regulatory element-binding protein 1, fructose-bisphosphate aldolase A, and peroxiredoxin-1. These up-regulated or down-regulated proteins are mostly related to lipid metabolism. The TNBG antitumor mechanism is probably to influence tumor lipid metabolism, resulting in accumulation of LDs in tumor cells.

关 键 词：TETRAZANBIGEN ANTITUMOR lipid metabolism PROTEOMICS 

分 类 号：R735.7[医药卫生—肿瘤]