氨基酸组成相同序列不同的小分子多肽的液相色谱-质谱联用分析  

Separation of small molecular peptides with same amino acid composition but different sequences by high performance liquid chromatography-electrospray ionization-mass spectrometry

作　　者：刘丽红[1] 陈子林[1] 

机构地区：[1]武汉大学药学院,武汉430072

出　　处：《中国科学（B辑）》2009年第8期800-805,共6页Science in China(Series B)

基　　金：国家自然科学基金(批准号:20775055;90817103);教育部留学回国人员启动基金(SRFforROCS;SEM);武汉大学珞珈特聘教授启动基金资助

摘　　要：多肽组学是近年来兴起的一门新型学科,质谱已成为多肽组学研究的强有力手段.然而,用于检测具有相同氨基酸组成但序列不同的多肽时,只能给出等同的分子离子峰,在多肽结构解析上受到一定限制.因此,发展色谱分离-质谱检测联用技术是分析具有相同氨基酸组成但序列不同的多肽的有效途径.本文建立了一种氨基酸组成相同序列不同的小分子多肽的反相液相色谱分离-电喷雾离子化质谱检测新方法.该方法采用高效液相色谱-质谱联用技术,以两种三肽Gly-Ser-Phe和Gly-Phe-Ser为模式样品对象,考察了小分子多肽在不同流动相组成、流动相添加剂及pH等条件下的液相色谱行为,并讨论其保留机理.研究结果表明,在最优化的实验条件下,该方法稳定性好,重现性高,为多肽组学研究中的多肽解析提供科学的分析方法.Mass spectrometry is the most universal and efficient tool for the structure identification of proteins and peptides in proteornics and peptidomics. However, there is a limitation for the identification of the peptides with the same amino acid composition but different sequences as these peptides have identical mass spectra of molecular ion. Thus, an efficient wag to solve this problem is to develop the methodology of high performance liquid chromatogra- phy-electrospray ionization-mass spectrometry （HPLC-ESI-MS）. A LC-ESI-MS method was developed for the sepa- ration of small molecular peptides with the same amino acid composition but different sequences. Two tripeptides of Gly-Ser-Phe and Gly-Phe-Ser were used as a model sample. The separation behavior has been investigated and the separation conditions have been optimized. This method could provide a scientific reference for peptide analysis in peptidomics and proteomics.

关 键 词：高效液相色谱 质谱 多肽 氨基酸 

分 类 号：Q517[生物学—生物化学]