PCBP1的新伴侣蛋白分子的研究(英文)  

Identification of novel partner proteins of PCBP1

作　　者：霍丽蓉[1,2] 申晨[1] Wei-na JU 邹俊华[1] 闫武[1] W. Ted BROWN Nanbert ZHONG 

机构地区：[1]北京大学医学遗传中心,北京100191 [2]山西医科大学基础医学院微生物学与免疫学教研室 [3]New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314, USA

出　　处：《北京大学学报（医学版）》2009年第4期402-408,共7页Journal of Peking University:Health Sciences

基　　金：Supported in part by the “985” program(985-2-035-39) of the Chinese Ministry of Education;“973” project(2007CB511902)of the Chinese Ministry of Sciences and Technology;National Nature Science Foundation(30671157);the NewYork State Office of Mental Retardation and Developmental Disabilities(NYSOMRDD)~~

摘　　要：Objective:PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hypothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1. Methods:To test our hypothesis,approaches of protein wal-king with a yeast two-hybrid system (Y2H),pulling down in yeasts,co-immunoprecipitation and immunofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial "walker" to search for its interaction partner(s). Results:Candidate proteins including MYL6,PECAM1,CSH1,RAB7,p57KIP2,ACTG1,RBMS1 and PSG4-like were identified with selection mediums and preceding methods. Conclusion:With these candidate protein molecules,some protein complexes associating with PCBP1 are proposed,which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.Objective：PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins （hnRNPs） that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hypothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1. Methods：To test our hypothesis,approaches of protein wal-king with a yeast two-hybrid system （Y2H）,pulling down in yeasts,co-immunoprecipitation and immunofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial ＂walker＂ to search for its interaction partner（s）. Results：Candidate proteins including MYL6,PECAM1,CSH1,RAB7,p57KIP2,ACTG1,RBMS1 and PSG4-like were identified with selection mediums and preceding methods. Conclusion：With these candidate protein molecules,some protein complexes associating with PCBP1 are proposed,which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.

关 键 词：Poly(C)结合蛋白质1 酵母菌 杂交 遗传 蛋白质结合 

分 类 号：R394.3[医药卫生—医学遗传学]