GC/MS,SIM定性定量分析云南野生毒蝇菌中的毒蝇碱  

Qualitative and quantitative determination of muscarine in amanita muscaria of Yunnan province with gas chromatography and mass spectrum

作　　者：林佶[1] 段志敏[1] 万玉萍[1] 沈其萍[1] 刘建辉[1] 

机构地区：[1]云南省疾病预防控制中心检验中心,昆明650032

出　　处：《中国卫生检验杂志》2009年第9期1988-1989,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研究探讨GC/MS测定毒蝇碱的分析方法。方法:通过毒蝇碱标准品建立毒蝇碱的特征离子谱库。用甲醇提取样品中的毒蝇碱,运用GC/MS的Xcalibur分析软件,与标准毒蝇碱的特征离子峰比较,定性定量测定毒蝇菌样品中的毒蝇碱含量。结果:云南野生毒蝇菌中毒蝇碱含量为25.6 mg/kg。结论:该方法简便、快速、灵敏度高、准确可靠。Objective：To study research analytical method of muscarine. Methods：Using muscarine standard sample to set up mass spectrum library. Muscarine in Amanita muscaria was extracted with methanol. Appling xcalibur sofeware of gas chromatography and mass spectrum instrument, we compared mass peak of amanita muscaria with mass peak of muscarine standard, qualitative and quantitative determination of muscarine in amanita muscaria. Results ：25.6 mg/kg muscarine content were determined in Amanita muscaria of Yunnan province. Conclusion：This method is fast, sensitive and accurate.

关 键 词：毒蝇碱 GC/MS 定性定量分析 

分 类 号：R155.5[医药卫生—营养与食品卫生学]