类植物乳杆菌的耐酸、耐胆盐及降胆固醇特性  

Acid and bile tolerance and cholesterol reduction ability of Lactobacillus paraplantarum

作　　者：刘长建[1] 刘秋[1] 姜波[1] 

机构地区：[1]大连民族学院生命科学学院,大连116600

出　　处：《微生物学报》2009年第9期1176-1179,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30671398);NSFC-KOSEF国际合作项目(30711140389)~~

摘　　要：【目的】研究了类植物乳杆菌Ⅱ32的酸、胆盐耐受性和胆固醇去除能力,并通过菌株生长和胆固醇去除的关系探讨可能的机理。【方法】菌株Ⅱ32生长在高胆固醇MRS培养基中,胆固醇的检测通过气相色谱法。检测菌株在不同生长阶段对胆固醇的去除情况。【结果】菌株Ⅱ32具有酸耐受性、胆盐耐受性和一定的胆固醇清除能力。菌株在pH2.0培养2h后仍能达到104cfu/mL;加胆盐(0.3%～0.4%)对菌株生长量达到OD值0.6的时间延迟在0.5h以内;热杀死的和休眠的细胞能去除很少的胆固醇,分别是5.64、5.90mg/g细胞干重,而生长的细胞去除的胆固醇达到16.98mg/g细胞干重。另外,研究表明胆固醇去除与菌体的生长有一定的相关性。【结论】菌株Ⅱ32去除胆固醇可能的机理是菌体对胆固醇的吸附及菌体在生长过程中对胆固醇的吸收利用,为此该菌株具有添加到食品中来降低血液胆固醇的潜能。[Objective] We studied the acid and bile tolerance of Lactobacillus paraplantarum Ⅱ 32, and examined the possible mechanisms of cholesterol reduction. [ Methods ] Strain Ⅱ 32 was incubated in MRS （de Man, Rogosa, Sharpe ） broth supplemented with cholesterol, and the cholesterol in medium was determined using gas chromatography. We examined cholesterol reducing ability under different conditions, and the relation between growth and cholesterol reduction of strain Ⅱ 32. [ Results] Strain Ⅱ 32 showed acid resistance, bile salt tolerance and the cholesterol-reducing ability. The results indicated that strain Ⅱ 32 survived at pH2 after 2 h culture and the living bacterial number could reach 10^4 cfu/mL. In addition, the growth of strain Ⅱ 32 was delayed less than 0.5 h in MRS broth with 0.3 % - 0.4 % bile salt, when the absorbance value both increased to 0.6 unit. Cholesterol removed by dead and resting cells were 5.64 and 5.90 mg/g of dry weight compared with growing cells, which was 16.98 mg/g of dry weight. Cholesterol removal was greatly associated with growth of the bacteria. The possible mechanisms for removal of cholesterol from media were proposed ： assimilation of cholesterol during growth, and binding of cholesterol to the cell surface. [ Conclusion] Strain Ⅱ 32 may be promising candidates for use as a dietary adjunct to lower serum cholesterol in vivo.

关 键 词：类植物乳杆菌 酸耐受性 胆盐耐受性 胆固醇清除 

分 类 号：TS201.2[轻工技术与工程—食品科学]