两株产抑菌物质乳酸菌的鉴定及系统发育分析  

Identification and Phylogenetic Analysis of Two Stains of Lactic Acid Bacteria with Antibacterial Functions

作　　者：贺银凤[1] 靳烨[1] 田建军[1] 吴敬[1] 张开屏[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《食品科学》2009年第19期232-235,共4页Food Science

基　　金：国家自然科学基金资助项目(29966003)

摘　　要：对分离自酸马奶酒中的两株产抑菌物质的乳酸菌BY-3-2、SQ-3-2进行分离鉴定及系统发育分析,以更准确地判定其属种。对菌株进行生理、生化特性鉴定及其16SrDNA序列分析。结果表明:菌株BY-3-2、SQ-3-2的生理、生化特性符合肠球菌属特征,其中BY-3-2菌株符合E.faecium的特征;SQ-3-2菌株符合E.durans的特征。通过16SrDNA序列进行系统发育分析,结果与生理生化鉴定相一致。In order to identify genus and species of two stains of lactic acid bacteria with antibacterial functions, BY-3-2 and SQ-3-2 were separated from Koumiss. Their physiological and biochemical characteristics and 16S rDNA sequence were analyzed. Results indicated that physiological and biochemical characteristics of BY-3-2 and SQ-3-2 were the same as those of Enterococcus. Moreover, strain BY-3-2 had the characteristics of E.faecium, and SQ-3-2 had the characteristics orE. durans, which was also supported by 16S rDNA sequence analysis.

关 键 词：乳酸菌 分离鉴定 16S-rDNA序列分析 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]