山葡萄色素的定性分析  

A Qualitative Analysis of Pigments in Vitis amurensis Rupr

作　　者：沈育杰[1] 赵景辉[1] 王金和[1] 王玉方[1] 刘彦[1] 常维春[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《特产研究》1998年第3期5-8,14,共5页Special Wild Economic Animal and Plant Research

摘　　要：采用纸层析法制取山葡萄色素中各纯组分。以浓盐酸：甲酸:水:24．9:23.7:51．4（V／V）为展开剂。分离出两个独立的非常明显的色谱带。根据0.1％盐酸甲醇液中显色，紫外-可见吸收光谱测定，与Alcl3反应，糖组分鉴定，色素的部分及完全水解，A44／A-max比值等综合研究结果推断，山葡萄色素两组分分别为芍药色素-3-葡萄糖苷（Pn-3-glu）和矢车菊素-3-葡萄糖苷（Cy-3-glu）。Pure components of pigments in Vitis amurensis Rupe. were prepared by the paperchromatography. Cone. Hcl - HCOOH - H2O(24. 9 : 23. 7: 51. 7, V/ V) being used as aspreader, two distinct bands were separated. Based on such overall studies as the developingin 0. 1% Hcl - MeOH Solution, detennination of ultraviolet - visible absorption spectrum,reaction with Alcl3, identification of carbohydrate components. partial and complete hydrolysis of pigments, calculating A440/ Amax ratio and so on, it can be deduced that the twocomponents of the pigments in Vitis amurensis is peonidin - 3 - glucoside (Pn - 3 - glu ) andcyanidin - 3 - glucoside(Cy - 3 - glu ), respectively.

关 键 词：山葡萄色素 定性分析 色素 光谱分析 酿酒 

分 类 号：TS202[轻工技术与工程—食品科学] TQ611[轻工技术与工程—食品科学与工程]