阳离子交换树脂分离纯化酶法合成的双果糖酐Ⅲ  

Separation and Purification of Difructose Anhydride Ⅲ Prepared by Enzymatic Synthesis Using Cation Exchange Resin

作　　者：赵萌[1] 张涛[1] 沐万孟[1] 江波[1] 卢峥嵘[1] 杜倩[1] 

机构地区：[1]食品科学与技术国家重点实验室(江南大学),江苏无锡214122

出　　处：《食品与发酵工业》2009年第9期134-136,141,共4页Food and Fermentation Industries

基　　金："十一五"国家863资助项目(2006AA10Z334)

摘　　要：采用DTF-02型阳离子交换树脂对酶法合成的双果糖酐Ⅲ进行了分离纯化,探讨了柱温、进样浓度、进样体积和流速对分离效果的影响。结果表明:选择柱温60℃、流速1 mL/min、进样体积5 mL、固形物含量20%的条件下,可以得到完全分开的双果糖酐Ⅲ和低聚果糖,此时收集含DFAⅢ的洗脱液,DFAⅢ含量在95%以上。The separation and purification of difructose anhydride prepared by enzymatic synthesis using cation exchange resin DTF-02 was studied. The influence of column temperature, sample concentration, sample volume and elution flow rate were discussed. The study showed that, when 5mL difruetose anhydride Ⅲ containing a soluble solid content of 20% , difructose anhydrideⅢ was completely separated from fructo-oligosaccharide with elution flow rate of 1 mL/min at 60℃. Under such condition, the fractions containing DFAⅢ were separated and determined by HPLC which showed DFA Ⅲ content was over 95%. The research laid theoretical foundations for large-scale separation and purification of difructose anhydride Ⅲ.

关 键 词：双果糖酐Ⅲ 低聚果糖 阳离子交换树脂 分离纯化 

分 类 号：TS202.3[轻工技术与工程—食品科学]