高活性几丁质酶产生菌的筛选及鉴定  

Isolating and identification of a higher enzyme activity strain BLC08609 producing chitinase

作　　者：鲜乔[1] 蒋家新[1] 张拥军[1] 李轶萍[1] 安晓欢[1] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018

出　　处：《中国计量学院学报》2009年第3期274-277,282,共5页Journal of China Jiliang University

基　　金：863计划专题项目(No.2007AA10Z342)

摘　　要：从四川、陕西、浙江等地食用菌栽培的土壤样品中,筛选到一株性能优良、产几丁质酶活力较强的菌株BLC08609,并结合菌落形态、生理生化指标和16S rDNA序列分析对其进行鉴定.结果显示,菌株BLC08609能以食用菌细胞壁(以几丁质为主)为唯一碳源生长,在菌株BLC08609个体形态和生理生化特性鉴定的基础上,通过16S rDNA测序鉴定,确定BLC08609为地衣芽孢杆菌(Bacillus licheniformis).A strain named BLC08609 with higher chitinase activity and high quality was isolated from soil samples in China. Its population shape and individual form were observed and identified from the aspects of physiological and biochemical characteristics and molecular biology. The result shows that the chitin can be the only carbon source for the BLC08609 to grow. Based on the identification of the population shape, the individual form and physiological and biochemical characteristics of the strain BLC08609, it is determined as Bacillus licheniformis through 16sDNA sequence appraisal. The experimental basis is provided for the selection and breeding of high yield strains of chitinase by this research.

关 键 词：几丁质酶 地衣芽孢杆菌 真菌细胞壁 

分 类 号：X172[环境科学与工程—环境科学]