酶电极法快速测定β(1→3)-D-葡聚糖含量的研究  

Study on determination the content of β(1→3)-D-glucan with enzyme biosensor

作　　者：楚杰[1] 刘可春[1] 张大伟[1] 毕春元[1] 何秋霞[1] 

机构地区：[1]山东省科学院生物研究所,山东济南250014

出　　处：《食品工业科技》2009年第10期324-325,329,共3页Science and Technology of Food Industry

摘　　要：采用酶电极法测定β(1→3)-D-葡聚糖的含量,结果表明,葡聚糖H2SO4水解的最佳时间为6h,水解液中和过程中产生的盐对测定结果无明显干扰作用,与硫酸-苯酚法相比,该方法具有快速、准确、稳定等优点,是目前测定β(1→3)-D-葡聚糖含量的一种理想方法。The inspected methods of β（1→3）-D-glucan was studied with glucose oxidase electrode.The results indicated that optimum hydrolyzed time of glucan was six hours with H2SO4.The salts of degraded liquids was not disturbed to determined outcome.This method has advantages of simplicity, rapidity and high accuracy with phenol -sulphate acid methods.

关 键 词：β(1→3)-D-葡聚糖 葡萄糖氧化酶 酶电极 

分 类 号：TS207.3[轻工技术与工程—食品科学]