一株Kefir源乳酸菌MA2的鉴定及对大鼠肠道菌群的影响  

Identification of Lactic Acid Bacteria MA2 Strain from Kefir Grains and Effect on Rat Intestinal Flora

作　　者：许女[1] 王艳萍[1] 习傲登[1] 李超[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《天津科技大学学报》2009年第5期1-5,共5页Journal of Tianjin University of Science & Technology

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A06)

摘　　要：对从西藏Kefir粒中分离出来的乳酸菌MA2进行了形态学、生理生化和16SrDNA分子生物学鉴定,确定其为植物乳杆菌.酸和胆盐耐受性实验显示,植物乳杆菌MA2在pH2.0、胆盐浓度为0.3%的情况下具有较好的耐受能力.动物实验证明,植物乳杆菌MA2显著促进了双歧杆菌和乳酸菌的生长,调节了肠道菌群的平衡,是一株潜在的、具有实际应用价值的益生菌.Lactic acid bacteria MA2 from Tibet keflr grain was identified as Lactobacillus plantarum by morphology, physiological and biochemistry methods and 16S rDNA sequencing. The results of tolerance of acid and bile salt show that MA2 strain has strong acid tolerance and bile salt tolerance at pH 2.0 and 0.3% oxgall concentration. The results of animal test indicate that MA2 can increase the amount of lactic acid bacteria and bifidobacteria in feces of rats,and improve the balance of intestinal flora. MA2 is a kind of potential probiotic with practical application.

关 键 词：KEFIR 植物乳杆菌MA2 肠道菌群 

分 类 号：Q93-3[生物学—微生物学]