产朊假丝酵母油脂高产菌株的诱变选育  

Breeding of Candida utilis strains with high yield of lipid by mutation

作　　者：全艳玲[1] 解生权[2] 王彤[1] 

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051 [2]辽宁经济职业技术学院,辽宁沈阳110122

出　　处：《中国酿造》2009年第11期32-33,共2页China Brewing

摘　　要：对产朊假丝酵母进行紫外诱变获得高产油脂突变菌株。紫外诱变后筛选出产油量最多菌种。通过正交试验得到产朊假丝酵母产油脂的最优条件:培养温度25℃,培养时间4d,接菌量10%,转速100r/min,在此条件下培养,紫外诱变10s后的产朊假丝酵母产油脂量可达到18%。Mutant Candida utilis strain with high yield of lipid was obtained by UV mutation. The optimum conditions for lipid production for mutant strain by orthogonal experiment were inoculation of 10%, culture at 25℃ with rotation at 100 r/min for 4 d. The maximum yield of lipid reached to 18% occurred at the strain treated with UV mutation for 10 s with optimum culture conditions.

关 键 词：酵母 筛选 紫外诱变 油脂 

分 类 号：Q93-331[生物学—微生物学]